Chromium X Series for Single Cell Analysis from 10x Genomics

Product Specs
ItemChromium X Series for Single Cell Analysis
Company10x Genomics
Price Get Pricing Get Quote for this Product
Catalog NumberChromium X
TypeSingle Cell Analysis System
ApplicationsSingle cell analysis
Research AreaOncology, Immunology, Neuroscience
Dimensions11.25” [28.6 cm] x 19” [48.3 cm] x 10.75” [27.3 cm] (W x D x H)
Operating Temperature64–82°F [18–28°C]
Electrical Requirements100–240 VAC, 50/60 Hz, 250 W

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10x Genomics

6230 Stoneridge Mall Road

Pleasanton, CA 94588-3260

United States

Phone: 1-925-401-7300
1-800-709-1208

Email: info@10xgenomics.com

Company Profile

Website: http://www.10xgenomics.com

(1) Activation of mTOR signaling in adult lung microvascular progenitor cells accelerates lung agingJournal of Clinical InvestigationDecember 15, 2023Emma C. Mason, Swapna Menon, Benjamin R. Schneider, Christa F. Gaskill, Maggie M. Dawson, Camille M. Moore, Laura Craig Armstrong, Okyong J. Cho, Bradley W. Richmond, Jonathan A. Kropski, et al.performed using the Chromium Single Cell 3′ Library and Gel Bead Kit (v3.1, 10x Genomics), and the Chromium X and libraries were sequenced on an Illumina NovaSeq 6000. Sequencing guidelines for scRNA-Seq included

(2) Conserved and divergent gene regulatory programs of the mammalian neocortexNatureDecember 13, 2023Nathan R. Zemke, Ethan J. Armand, Wenliang Wang, Seoyeon Lee, Jingtian Zhou, Yang Eric Li, Hanqing Liu, Wei Tian, Joseph R. Nery, Rosa G. Castanon, et al.tubes for loading onto the Chromium Next GEM Chip J system and carried out droplet generation using the Chromium X microfluidic system (10x Genomics). Reverse transcription and cell barcoding were performed inside the

(3) Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screensNature GeneticsNovember 9, 2023Sean R. McCutcheon, Adam M. Swartz, Michael C. Brown, Alejandro Barrera, Christian McRoberts Amador, Keith Siklenka, Lucas Humayun, Maria A. ter Weele, James M. Isaacs, Timothy E. Reddy, et al.expanded for 10 days and then stained and sorted for Thy1.1+ cells. Sorted cells were loaded into the Chromium X for a targeted recovery of 2 × 104 cells per donor and treatment according to the Single Cell 5′-High-Throughput

(4) Mapping the cellular and molecular landscape of cardiac non-myocytes in murine diabetic cardiomyopathyiScienceOctober 20, 2023Charles D. Cohen, Miles J. De Blasio, Gabriella E. Farrugia, Malathi S.I. Dona, Ian Hsu, Darnel Prakoso, Helen Kiriazis, Crisdion Krstevski, David M. Nash, Mandy Li, et al.Dickinson, USA",Aria™ Fusion Single cell library preparation using a 10X Chromium controller,10X Genomics,Chromium X Series Sequencing was performed on an Illumina NovaSeq 6000 system,Illumina,NovaSeq 6000

(5) Droplet-based single-cell joint profiling of histone modifications and transcriptomesNature Structural & Molecular BiologyOctober 1, 2023Yang Xie, Chenxu Zhu, Zhaoning Wang, Melodi Tastemel, Lei Chang, Yang Eric Li, Bing Rennuclei and barcoded beads are then coencapsulated into droplets with a microfluidic device (10x Genomics Chromium X controller). Two types of oligonucleotides with the same set of barcodes are embedded in the beads:

(6) Therapeutic Effects of Upadacitinib on Experimental Autoimmune Uveitis: Insights From Single-Cell AnalysisInvestigative Opthalmology & Visual ScienceSeptember 15, 2023Zhaohao Huang, Qi Jiang, Junjie Chen, Xiuxing Liu, Chenyang Gu, Tianyu Tao, Jianjie Lv, Zhaohuai Li, Zuohong Li, Wenru Susample processing, library preparation, and instrument and sequencing settings on the 10x Genomics Chromium X platform, we conducted scATAC-seq data reads aligned to the GRCh38 (hg38) reference genome and quantification

(7) Thymic epithelial cell fate and potency in early organogenesis assessed by single cell transcriptional and functional analysisFrontiers in ImmunologyJuly 25, 2023Alison Mary Farley, An Chengrui, Sam Palmer, Dong Liu, Anastasia I. Kousa, Paul Rouse, Viktoria Major, Joanna Sweetman, Jan Morys, Andrea Corsinotti, et al.and loaded on a Chip G (10X Genomics). Cell-bead encapsulation was performed running the chip in a Chromium X instrument (10X Genomics). Upon encapsulation, gene expression and multiplexing libraries were prepared

(8) Venetoclax treatment in patients with cancer has limited impact on circulating T and NK cellsBlood AdvancesJune 27, 2023Charis E. Teh, Hongke Peng, Meng-Xiao Luo, Tania Tan, Marie Trussart, Lauren J. Howson, Chong Chyn Chua, Christine Muttiah, Fiona C Brown, Matthew E. Ritchie, et al.the TotalSeq-C Human Cocktail (Biolegend, #399905). Single cells were captured and barcoded using the Chromium X Instrument (10x Genomics) and libraries were prepared according to the manufacturer instructions. Analysis

(9) Orthogonal CRISPR screens to identify transcriptional and epigenetic regulators of human CD8 T cell functionbioRxivMay 5, 2023Sean R. McCutcheon, Adam M. Swartz, Michael C. Brown, Alejandro Barrera, Christian McRoberts McRoberts Amador, Keith Siklenka, Lucas Humayun, James M. Isaacs, Timothy E. Reddy, Smita Nair, et al.expanded for 10 days and then stained and sorted for Thy1.1+ cells. Sorted cells were loaded into the Chromium X for a targeted recovery of 2 × 104 cells per donor and treatment according to the Single Cell 5’-High-Throughput

(10) Pioneer factor ASCL1 cooperates with the mSWI/SNF complex at distal regulatory elements to regulate human neural differentiationGenes & DevelopmentMarch 17, 2023Oana Păun, Yu Xuan Tan, Harshil Patel, Stephanie Strohbuecker, Avinash Ghanate, Clementina Cobolli-Gigli, Miriam Llorian Sopena, Lina Gerontogianni, Robert Goldstone, Siew-Lan Ang, et al.concentration of 2000 cells/mL. This suspension was then counted. Ten-thousand cells were loaded onto the Chromium X (10X Genomics) to generate single-celled gel beads in emulsion (GEMs) for library preparation. Dual-index

(11) Efficient isolation of rare B cells using next-generation antigen barcodingFrontiers in Cellular and Infection MicrobiologyMarch 10, 2023Jonathan Hurtado, Claudia Flynn, Jeong Hyun Lee, Eugenia C. Salcedo, Christopher A. Cottrell, Patrick D. Skog, Dennis R. Burton, David Nemazee, William R. Schief, Elise Landais, et al.from 1.6x106 single B cells, was recently obtained using the emulsion microfluidics-based 10x Genomics Chromium X platform (Jaffe et al., 2022). In 2017, CITE-seq ( C ellular I ndexing of T ranscriptomes and E pitopes

(12) Single-cell RNA-seq methods to interrogate virus-host interactionsSeminars in ImmunopathologyJanuary 1, 2023Kalani Ratnasiri, Aaron J. Wilk, Madeline J. Lee, Purvesh Khatri, Catherine A. Blishparticularly increasing the capacity to profile thousands of cells. Recently, 10X Genomics developed the Chromium X controller that pushes high-throughput scRNA-seq sequencing from 10 to 60 K cells per sample by adding

(13) BCL11B depletion induces the development of highly cytotoxic innate T cells out of IL-15 stimulated peripheral blood αβ CD8+ T cellsOncoImmunologyDecember 31, 2022Hannes Forkel, Piotr Grabarczyk, Maren Depke, Sascha Troschke-Meurer, Stefan Simm, Elke Hammer, Stephan Michalik, Christian Hentschker, Björn Corleis, Lucie Loyal, et al.each experimental condition of a single donor were used for single-cell library preparation using the Chromium X Series (10X Genomics, USA) and the Chromium Next GEM Single Cell 3’ GEM, Library and Gel Bead Kit 3.1

(14) Functional antibodies exhibit light chain coherenceNatureOctober 26, 2022David B. Jaffe, Payam Shahi, Bruce A. Adams, Ashley M. Chrisman, Peter M. Finnegan, Nandhini Raman, Ariel E. Royall, FuNien Tsai, Thomas Vollbrecht, Daniel S. Reyes, et al.its data. V(D)J sequences were obtained using the 10x Genomics Immune Profiling Platform, using six Chromium X HT chips and standard manufacturer methods. cDNA libraries were sequenced on the NovaSeq 6000 platform

(15) CXCR4+ Treg cells control serum IgM levels and natural IgM autoantibody production by B-1 cells in the bone marrowJournal of Experimental MedicineJuly 4, 2022Shlomo Elias, Rahul Sharma, Michael Schizas, Izabella Valdez, Sham Rampersaud, Sun-Mi Park, Paula Gonzalez-Figueroa, Quan-Zhen Li, Beatrice Hoyos, Alexander Y. Rudenskyantibodies (BioLegend), diluted (1.5 × 103 cells/μl in PBS, 0.04% BSA), pooled, and processed using Chromium X platform (10x Genomics). 50,000 and 44,432 CD4conv T cells and 46,230 and 20,827 Treg cells from spleen

(16) Simple oligonucleotide-based multiplexing of single-cell chromatin accessibilityMolecular CellOctober 21, 2021Kaile Wang, Zhenna Xiao, Yun Yan, Rui Ye, Min Hu, Shanshan Bai, Emi Sei, Yawei Qiao, Hui Chen, Bora Lim, et al.microdroplet platforms that have enabled sequencing up to 1M cells in one experimental run (eg. 10X Genomics Chromium X) compared to 10K cells which is the maximum on current platforms. Finally, we expect that SNuBar will

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Chromium X Series for Single Cell Analysis from 10x Genomics

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10x Genomics

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Enhancing the Characterization of Cell Types and States with Single Cell Multiomics

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Chromium X Series for Single Cell Analysis from 10x Genomics

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